SYBR Green II RNA gel stain *10,000× concentrate in DMSO*

 

Name

Sybr Green II RNA gel stain 10000× concentrate in DMSO

CAT#

D007

Price

65$/50μL

Pack Size

50μL/100μL

Solvent

DMSO

Ex   (nm)

497

Em   (nm)

520

Storage

4ºC, desiccation and avoid light

Shelf   Life

12 months

 

SYBR Green II RNA gel stain is one of the most sensitive dyes for detecting RNA in electrophoretic gels. As little as 100 pg RNA or single-stranded DNA per band can be detected in a SYBR Green II-stained agarose or polyacrylamide gel at 254 nm and a SYBR Green gel stain photographic filter. Even with 300 nm transillumination, as little as 500 pg RNA per band can be detected.

 

SYBR Green II RNA gel stain is significantly more sensitive than ethidium bromide. The remarkable sensitivity of SYBR Green II RNA gel stain for detecting RNA can be attributed to several factors, including superior fluorescence quantum yield, binding affinity and fluorescence enhancement. Although it is not selective for RNA staining, this dye exhibits a higher quantum yield when bound to RNA (~0.54) than to double-stranded DNA (~0.36). This property is somewhat unusual among nucleic acid stains; most show far greater quantum yields and fluorescence enhancements when bound to double-stranded nucleic acids. The fluorescence quantum yield of the RNA/SYBR Green II complex is more than seven times greater than that of the RNA/ethidium bromide complex (~0.07). The affinity of SYBR Green II RNA gel stain for RNA is also higher than that of ethidium bromide, and its fluorescence enhancement upon binding RNA is well over an order of magnitude greater. Because SYBR Green II RNA gel stain has a low intrinsic fluorescence, there is no need to destain gels to remove free dye.

 

The fluorescence of RNA/SYBR GreenII complexes is not quenched in the presence of urea or formaldehyde, eliminating the need to wash these denaturants out of gels prior to staining. In addition, staining agarose/formaldehyde gels with SYBR Green II RNA gel stain does not interfere with transfer of RNA to filters or subsequent hybridization in Northern blot analysis as long as 0.1%–0.3% SDS is included in prehybridization and hybridization buffers.


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