SYBR Green I nucleic acid gel stain *10,000× concentrate in DMSO*

Name

SYBR Green I nucleic acid gel stain *10,000× in DMSO* 

CAT#

D001

Price

65$/50μL

Pack Size

50μL/100μL

Solvent

DMSO

Ex   (nm)

497

Em   (nm)

525

Storage

4ºC, desiccation and avoid light

Shelf   Life

12 months

SYBR Green I nucleic acid gel stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels. With 300 nm transillumination, as little as 60 pg dsDNA per band can be detected with SYBR Green I stain. Less than 20 pg of dsDNA can be detected in a single band of a SYBR Green I stained gel using 254 nm.

This sensitivity is at least 25 times greater than ethidium bromide. We also have found that SYBR Green I stain is also a very sensitive stain for oligonucleotides, allowing the detection of as little as 1–2 ng of a synthetic 24-mer on 5% polyacrylamide gels—a sensitivity that is 50–100 times greater than can be achieved with ethidium bromide.  

The sensitivity for detecting single-stranded DNA and RNA is somewhat lower (approximately 100 to 300 pg per band using 254 nm epi-illumination), making SYBR Green I stain ideal for detecting dsDNA in complex solutions, where ssDNA or RNA in the sample may obscure the results, such as apoptosis ladders. SYBR Green I stain exhibits exceptional affinity for DNA and a large fluorescence enhancement upon DNA binding at least an order-of-magnitude greater than that of ethidium bromide.

Also, the fluorescence quantum yield of the DNA/SYBR Green I complex (~0.8) is over five times greater than that of DNA/ethidium bromide (~0.15). SYBR Green I stain quickly penetrates both agarose and polyacrylamide gels and has negligible background fluorescence in the absence of DNA, allowing a rapid staining procedure that requires no destaining step prior to photography.

The exceptional sensitivity of SYBR Green I stain makes it useful for many applications where the amount of DNA is limiting, including the detection of low–cycle number and low–target number DNA amplification products;4 the detection and restriction analysis of low–copy number DNA and RNA vectors and of cosmids, plasmids, and phage DNA from cultures with low cell numbers; and the detection of products of nuclease protection and bandshift assays. SYBR Green I stain’s superior sensitivity has even allowed the replacement of radioisotopes in some applications.  Because of SYBR Green I stain’s strong DNA binding affinity, it can be used to stain DNA before electrophoresis (prestaining), as well as after electrophoresis (poststaining)  and has also been used to stain DNA separated by capillary electrophoresis.  Gels can be precast with SYBR Green I stain; however, we have found the greatest sensitivity is achieved by poststaining. Furthermore, our researchers have found that the presence of SYBR Green I stain bound to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I  and does not interfere with Southern blotting techniques. 

  • Email : info@bridgen.cn
  • Tel : +86-010-57208608
designed by instrue
Copyright©2019 Bridgen Biotech Co., Ltd.