DuRed® nucleic acid gel stain *10,000× in water


  Name

  DuRed® nucleic acid gel stain *10,000× in water

  CAT#

  D009

  Price

  60$

  Pack Size

  500μL

  Solvent

  DMSO/H2O

  Ex (nm)

  304

  Em (nm)

  608

  Storage

  4ºC, desiccation and avoid light

  Shelf Life

  12 months

 

 

Description

 

DuRed® Nucleic Acid Gel Stain is a kind of new generation of fluorescent nucleic acid gel stain designed to replace the highly toxic ethidium bromide (EtBr). A series of safety tests have confirmed that DuRed® is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining.

 

DuRed®Nucleic Acid Gel Stain are highly sensitive than EtBr either as precast gel stains or post gel stains. DuRed® and EB have virtually the same spectra, so you can directly replace EB with Dured without changing your existing imaging system. DuRed® can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining.

 

DuRed® Nucleic Acid Gel Stain 10,000X in water is a concentrated DuRed solution that can be diluted 10,000 times for use in precast gel staining for ~3,300 times for use in post gel staining according to the procedures described below. One vial(0.5ml) of 10,000X solution can be used to prepare at 100 precast minigels or post-stain at least 100 minigels.

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Figure 1.Normalized excitation and emission spectra of DuGreen (green) and DuRed (red) in the presence of dsDNA in PBS buffer.

 

Features

 

Ø Safety: Nonmutagenic and noncytotoxic

Ø Easy disposal: Safe to dispose in the drain

Ø Compatibility: Spectrally compatible with existing instruments

Ø Sensitivity: Higher signal but lower background

Ø Stability: can be stored at RT and microwavable

 

Tip: If you are using image system with UV, please choose Product A; by using image system with visible light excitation, please choose Product B.


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