DuGreen® nucleic acid gel stain *10,000× in water


  Name

  DuGreen® nucleic acid gel stain *10,000× in water

  CAT#

  D011

  Price

  $60

  Pack Size

  500μL

  Solvent

  DMSO/H2O

  Ex (nm)

  471

  Em (nm)

  540

  Storage

  4ºC, desiccation and avoid light

  Shelf Life

  12 months

 

Description
DuGreen Nucleic Acid Gel Stain is a kind of new generation of fluorescent nucleic acid gel stain designed to replace the highly toxic ethidium bromide (EtBr). The Ames test confirmed that DuGreen are nonmutagenic at concentrations well above their working concentrations used for gel staining. DuGreen  Nucleic Acid Gel Stain are highly sensitive than EtBr either as precast gel stains or post gel stains.

Dugreen has UV absorption between 250nm and 300nm and a strong absorption peak centered around 500nm and is compatible with either a 254nmUV transilluminator or a gel reader equipped with visible light excitation(such as a 488nm laser-based gel scanner or a Dark Reader).

DuGreen Nucleic Acid Gel Stain 10,000X in DMSO is a concentrated DuGreen solution that can be diluted 10,000 times for use in precast gel staining for ~3,300 times for use in post gel staining according to the procedures described below. One vial(0.5ml) of 10,000X solution can be used to prepare at 100 precast minigels or post-stain at least 100 minigels.

Gel staining with DuGreen is compatible with downstream applications such as gel extraction and cloning. DuGreen is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.

 

image.png

Figure 1.Normalized excitation and emission spectra of DuGreen (green) and DuRed (red) in the presence of dsDNA in PBS buffer.

 

Features

 

Ø Safety: Nonmutagenic and noncytotoxic

Ø Easy disposal: Safe to dispose in the drain

Ø Compatibility: Spectrally compatible with existing instruments

Ø Sensitivity: Higher signal but lower background

Ø Stability: can be stored at RT and microwavable

 

Tip: If you are using image system with UV, please choose DuRed; by using image system with visible light excitation, please choose DuGreen.

 



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